Heterogenous degradation of myofibrillar proteins.
نویسندگان
چکیده
منابع مشابه
Degradation of myofibrillar proteins by trypsin-like serine proteinases.
The effects of the Ca2+-activated cysteine proteinase, the rat trypsin-like serine proteinase and bovine trypsin on myofibrillar proteins from rabbit skeletal muscle are compared. 2. Myofibrils that had been treated at neutral pH with the Ca2+-dependent proteinase and with the rat enzyme were (a) analyzed by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and (b) examined in the elec...
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The objective of this study was to investigate the potential contribution of caspase-3 to meat postmortem tenderisation by examining the role of caspase-3 in the degradation of myofibrillar proteins and disruption of myofibril structure in vitro. Myofibrillar protein prepared from chicken muscle was incubated with EDTA or EDTA plus caspase-3 at 25°C for 16h and used for detecting muscle protein...
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In order to examine the role of muscle lysosomes in degradation of intracellular protein, rat gastrocnemii were labeled in viuo by intramuscular injections of [ethyZ-2-3H~-ethylmaleimide. A rapid loss of radioactivity was observed and is attributed to proteolysis of modified proteins because 2-S-cysteino-(N-ethyl)-succinimide appeared in the urine. Mitochondrial-lysosoma1 fractions prepared fro...
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T he growth and hypertrophy of left ventricular myocardium in response to pressure or volume overload or segmental loss of functional myocardium was once considered a purely beneficial, structural adaptation to reduce or normalize systolic and/or diastolic wall stress.' This concept has been challenged, however, by a growing body of evidence indicating that most forms of left ventricular hypert...
متن کاملDegradation of myofibrillar proteins by extractable lysosomal enzymes and m-calpain, and the effects of zinc chloride.
A study was conducted to examine the effects that physiological levels of m-calpain (calpain requiring millimolar concentrations of Ca2+) extract and a lysosomal extract have on myofibrillar proteins in vitro, and the effects that zinc has on inhibiting proteolysis by these extracts. During a 22-h incubation period, the lysosomal extract degraded myosin heavy chain, alpha-actinin, desmin, tropo...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1984
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.48.2583